CD11b Antibody

SKU: orb1270680

Description

CD11b Antibody

Research Area

,Immunology,Neuroscience,Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Reactivity: Human
• Application Notes:
  For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:500For FACS starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This CD11b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the N-terminal region of human CD11b.
• Target: ITGAM
• Molecular Weight: 127 kDa
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, ITGAM, CD11B, CR3A

Western Blot at 1:2000 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.

Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Antibody staining D11b in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Antibody staining D11b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:500 dilution + human spleen lysate Lysates/proteins at 20 ug per lane.

Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in A549, K562 cell line lysates (35 ug/lane).

Quick Database Links

Gene Symbol

ITGAM

UniProt

P11215

RefSeq:P11215