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Description
Research Area
Images & Validation
−![CD273 [PD-L2] Recombinant Protein](/images/quality_badge_proteins.png)
| Application Notes |
|---|
Key Properties
−| Source | CHO cells |
|---|---|
| Target | PDCD1LG2 |
| Tag | Fc Tag |
| Purity | ≥98% (SDS-PAGE). Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza). |
| Protein Sequence | The extracellular domain of human CD273 [PD-L2] (aa 20-219) is fused to the N-terminus of the Fc region of human IgG1. |
Storage & Handling
−| Storage | Stable for at least 1 year after receipt when stored at -20˚°C. Working aliquots are stable for up to 3 months when stored at -20˚°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from 0.2um-filtered solution in PBS. Reconstitute with 100 μl sterile water. Add 1X PBS to the desired protein concentration. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human PDL2 Protein, His Tag [orb757453]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 23.4 kDa after removal of the signal peptide.The apparent molecular mass of PDL2-His is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μgRecombinant human PDCD1LG2 protein, C-His-Avi (HEK293) [orb1516350]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result. kDa
100 μgCD273 Recombinant Protein [orb1227912]
WB
> 95% as determined by SDS-PAGE more than 90% measured by SEC-HPLC.
49.2 kDa
HEK293 cells
0.1 mgCD273 Recombinant Protein [orb1227913]
WB
> 95% as determined by SDS-PAGE.
23.4 kDa
HEK293 cells
0.1 mgPD-L2 Recombinant Protein [orb1227729]
ELISA, WB
> 95% as determined by reduced SDS-PAGE.Less than 1.0 EU per ug by the LAL method.
48.9 kDa
HEK293 cells
0.1 mg

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