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Description
Research Area
Images & Validation
−| Tested Applications | FC |
|---|---|
| Dilution Range | FACS: 1:100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | OKT3 |
| Target | CD3E |
| Molecular Weight | 23147 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−CD3 Intracellular domain [orb1294242]
IF, IHC, WB
Human, Mouse, Rabbit
Rabbit
Polyclonal
Unconjugated
100 μl, 25 μl

Quality Guarantee
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Overlay histogram showing huuman peripheral blood lymphocytes stained with CD3 antibody (green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1: 50 dilution) for 60min at 37°C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40min at 37°C. Isotype control antibody (blue line) was mouse IgG2a (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_000724.1 |
|---|
Documents Download
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CD3 Antibody (orb167090)
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