CD3 Antibody

SKU: orb2638133

Description

CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.

Images & Validation

• Tested Applications: FACS, IF
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD3 antibody for a specific application should be determined by researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: B-B12
• Immunogen: Human T cell leukemia cells were used as immunogen for the CD3 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: The CD3 antibody is available for research use only.
• Disclaimer: For research use only

Immunofluorescent staining of FFPE human Jurkat cells with CD3 antibody (green, clone B-B12) and Reddot nuclear stain (red).

Flow cytometry testing of human Jurkat cells with CD3 antibody (clone B-B12); Red = isotype control, Blue = CD3 antibody.

SDS-PAGE analysis of purified, BSA-free CD3 antibody (clone B-B12) as confirmation of integrity and purity.

Quick Database Links

UniProt

P07766