CD3 Antibody

SKU: orb385668

Description

The CD3 complex mediates signal transduction. [UniProt]

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Immunology,Epigenetics

Images & Validation

• Tested Applications: FACS, Functional Studies, IF
• Dilution Range: Functional studies (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the CD3 antibody for a specific application should be determined by researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG3, kappa
• Clone No.: RIV9
• Immunogen: Human peripheral lymphocytes were used as immunogen for the CD3 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: CD3 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: The CD3 antibody is available for research use only.
• Disclaimer: For research use only

Immunofluorescent staining of FFPE human Jurkat cells with CD3 antibody (green, clone RIV9) and Reddot nuclear stain (red).

Flow cytometry testing of human Jurkat cells with CD3 antibody (clone RIV9); Red = isotype control, Blue = CD3 antibody.

SDS-PAGE analysis of purified, BSA-free CD3 antibody (clone RIV9) as confirmation of integrity and purity.

Immunofluorescent staining of methanol-fixed human tonsil tissue cryosection with CF488A-CD3 antibody (clone RIV9, green) and DAPI nuclear stain (blue).

Flow cytometry testing of lymphocyte-gated human PBM cells with CF488A-CD3 antibody (clone RIV9); Gray = unstained, Green = CD3 antibody.

Quick Database Links

UniProt

P07766