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CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes) Antibody

SKU: orb2315831

Description

CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes) Antibody

Research Area

,AKT Signaling|Apoptosis|Autophagy|Cardiovascular|Cytokine Signaling|Immunology

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC, WB
Dilution RangeFlow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
ReactivityHuman
Application Notes
Positive Control: Jurkat cells. Human tonsil, spleen or thymus.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG2b
Clone No.CD40LG/2761
ImmunogenRecombinant fragment (around aa 108-261) of human CD40LG protein (exact sequence is proprietary)
TargetCD40LG
Source/Expression SystemBioreactor
Molecular Weight36kDa
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
ConcentrationPurified Ab WITHOUT BSA and Azide at 1.0mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD154; gp39; hCD40L; HIGM1; IGM; IMD3; T B cell activating molecule; T BAM; T-cell antigen Gp39; TNF-related activation protein; TrAP; Tumor necrosis factor ligand superfamily member 5 (TNFSF5)

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 660.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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