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CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes) Antibody

SKU: orb751205

Description

Mouse monoclonal antibody against CD40LG conjugated to Biotin

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Product Categories/Antibodies,AKT Signaling|Apoptosis|Autophagy|Cardiovascular|Cytokine Signaling|Immunology

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC, WB
Dilution RangeELISA (For coating, order antibody without BSA); Flow Cytometry (2-4ug/million cells); ,Immunofluorescence (2-4ug/ml); Western Blot (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
ReactivityHuman
Application Notes
Positive Control: Jurkat cells. Tonsil, Spleen or Thymus

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG2b
Clone No.CD40LG/2761
ImmunogenRecombinant fragment (around aa 108-261) of human CD40LG protein (exact sequence is proprietary)
TargetCD40LG
Source/Expression SystemBioreactor
Molecular Weight36kDa
ConjugationUnconjugated

Storage & Handling

StorageAntibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Form/Appearance200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Buffer/PreservativesAntibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
ConcentrationPurified Ab conjugated to Biotin
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Anti-CD154 antibody, anti-gp39 antibody, anti-hCD40L antibody, anti-HIGM1 antibody, anti-IGM antibody, anti-IMD3 antibody, anti-T B cell activating molecule antibody, anti-T BAM antibody, anti-T-cell antigen Gp39 antibody, anti-TNF-related activation protein antibody, anti-TrAP antibody, anti-Tumor necrosis factor ligand superfamily member 5 (TNFSF5) antibody

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 660.00
Free Secondary Antibody (20 ul)0/0

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