CD44 Antibody

SKU: orb97676

Description

Mouse monoclonal to CD44.This CD44 monoclonal antibody is generated from mouse immunized with CD44 recombinant protein.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Stem Cells,Epigenetics,Primary Antibodies;Cancer;Immunology;Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IHC-P: 1:10-50, WB: 1:2000, IF/ICC: 1:10-50, FACS: 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a ,K
• Clone No.: Hermes-3
• Target: CD44
• Molecular Weight: 81538
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CD44 antibody, anti LHR antibody, anti MDU2 antibody, anti MDU3 antibody, anti MIC4 antibody, anti CD44 antigen antibody, anti CDw44 antibody, anti Epican antibody, anti Extracellular matrix receptor III antibody, anti GP90 lymphocyte homing/adhesion receptor antibody, anti HUTCH-I antibody, anti Heparan sulfate proteoglycan antibody, anti Hermes antigen antibody, anti Hyaluronate receptor antibody, anti Phagocytic glycoprotein 1 antibody, anti Phagocytic glycoprotein I antibody

All lanes: Anti-CD44 Antibody at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HUVEC whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 82 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

CD44 antibody confocal immunofluorescent analysis with hela cell. 0.01 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits red fluorescence. DAPI was used to stain the cell nuclear (blue).

CD44 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD44 antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Staining CD44 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Staining CD44 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Staining CD44 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Staining CD44 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Quick Database Links

Gene Symbol

CD44

UniProt

P16070

RefSeq (Protein):NP_001001390.1, NP_001001389.1, NP_001189486.1, NP_001001391.1, NP_001189485.1, NP_001001392.1, NP_001189484.1, NP_000601.3