CD57 / HNK-1 Antibody

SKU: orb1825920

Description

Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2, lambda
• Clone No.: NK1/7566
• Immunogen: A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the CD57 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the CD57 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CD57 antibody is available for research use only.
• Disclaimer: For research use only

Western blot testing of human Daudi cell lysate with CD57 antibody (clone NK1/7566). Predicted molecular weight ~38 kDa.

SDS-PAGE analysis of purified, BSA-free CD57 antibody (clone NK1/7566) as confirmation of integrity and purity.

IHC staining of FFPE human lymph node tissue with CD57 antibody (clone NK1/7566). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

Q9P2W7