CD57 / HNK-1 Antibody

SKU: orb749519

Description

Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics

Images & Validation

• Tested Applications: IF, IHC-P
• Dilution Range: Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD57 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgM, kappa
• Clone No.: HNK-1 or Leu-7
• Immunogen: Membrane antigen from HSB-2 cells was used as the immunogen for the CD57 antibody.
• Purity: PEG precipitation
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the CD57 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CD57 antibody is available for research use only.
• Disclaimer: For research use only

IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with CD57 antibody (clone HNK-1).

Western blot testing of K562 cell lysate with CD57 antibody (clone HNK-1). Predicted molecular weight: ~38/110kDa (unmodified/glycosylated).

Quick Database Links

UniProt

Q9P2W7