CD73 Rabbit Polyclonal Antibody

SKU: orb2931

Description

CD73 Rabbit Polyclonal Antibody

Research Area

Leukocyte Recruitment, Immunology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Guinea pig, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD73/NT5 (151-250/574aa)
• Target: Nt5e
• Molecular Weight: 70 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NT5; 5' NT; 5' nucleotidase(CD73); 5' nucleotidase precursor; 5' nucleotidase, ecto; 5' nucleotidase, ecto(CD73); 5'-NT; 5'-nucleotidase; 5'-nucleotidase; 5NTD_HUMAN; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN antibody eNT; NT antibody NT5; NT5E; NTE; Purine 5 Prime Nucleotidase.

Blank control (blue line): Raji (blue). Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (orb2931), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol.

Sample: HepG2 (human) cell Lysate at 40 ug, Primary: Anti-CD73 (orb2931) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 63 kD.

Sample: Kidney (mouse)l Lysate at 40 ug, Primary: Anti-CD73 (orb2931) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 65 kD.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: A549 (Human) Cell Lysate at 30 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-CD73 (orb2931) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63/71 kD, Observed band size: 65 kD.

Quick Database Links

Gene Symbol

Nt5e

UniProt

P21589