Chemiluminescent Western Blot Kit

SKU: orb342274

Description

Chemiluminescent Western Blot Kit

Research Area

Product Categories/Products/ELISA Kits,Detection Kit Type: Chemiluminescent Western Blot Kit

Images & Validation

• Tested Applications: WB
• Application Notes:
  Use Rockland Immuno-chemicals’ Anti-DYKDDDDK (FLAG) Chemiluminescent Kit for Western Blotting for detection of FLAG -tagged recombinant proteins by Western blot. This kit is useful for both ''Western blotting'' and ''dot blotting'' methods. After protein separation and transfer, the membrane is probed with Anti-DYKDDDDK (FLAG). Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.

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Key Properties

• Conjugation: Unconjugated
• Purity: This Chemiluminescent Western Blot Kit allows for the detection of FLAG -tagged recombinant protein present in cell lysates provided by the user.

Storage & Handling

• Storage: See kit insert for complete instructions.
• Expiration Date: Please enquire.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

Flag Western Blot, Western Blotting Kit, Chemiluminescent Flag Kit, Immunoblotting Kit, FLAG, HRP Anti FLAG, DDK, FLAG, FLAG antibody, anti-DDK, DDK antibody