Chicken IgG F(ab')2 Antibody Peroxidase Conjugated

SKU: orb346915

Description

Chicken IgG F(ab')2 antibody (Peroxidase)

Research Area

Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:40,000 - 1:90,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Reactivity: Gallus
• Application Notes:
  Anti-Chicken IgG F(ab')2 Peroxidase conjugate has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Chicken IgG F(ab')2 fragment
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab’)2 and Chicken Serum. No reaction was observed against Chicken IgG F(c).
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Concentration: 1.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Chicken IgG F(ab')2 Antibody Peroxidase Conjugation, rabbit anti-Chicken IgG Fab2 Fragment HRP Conjugated Antibody, rabbit anti-Chicken IgY F(ab')2 HRP

Western Blot of Unconjugated Anti-Chicken IgG F(ab')2 (RABBIT) Antibody (p/n orb346907). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n orb2652774). Lane 2: Chicken IgG F(c) Fragment (p/n orb346051). Lane 3: Chicken IgG F(ab) Fragment (p/n orb2652771). All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(ab')2 (RABBIT) Antibody (p/n orb346907) 1:5000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347654) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.