F(ab')2 Chicken IgG F(ab')2 Antibody Peroxidase Conjugated

SKU: orb346747

Description

F(ab')2 Chicken IgG F(ab')2 antibody (Peroxidase)

Research Area

Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Reactivity: Gallus
• Application Notes:
  This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG F(ab')2
• Immunogen: Chicken IgG F(ab')2 fragment
• Purity: This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab’)2 and Chicken Serum. No reaction was observed against Chicken IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Concentration: 10.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit F(ab')2 anti-Chicken IgG F(ab')2 Antibody peroxidase Conjugated, Rabbit F(ab')2 anti-Chicken IgG Fab2 fragment HRP conjugated antibody, Rabbit F(ab')2 anti-Chicken IgY F(ab')2 Antibody peroxidase Conjugated, Rabbit F(ab')2 anti-Chicken IgY Fab2 fragment HRP conjugated antibody