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Description
Research Area
Images & Validation
−| Tested Applications | FC, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This EHD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-48 amino acids from the N-terminal region of human EHD3. |
| Target | EHD3 |
| Molecular Weight | 61 kDa |
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−EHD3 Antibody [orb745908]
FC, ICC, IF, IHC, WB
Human, Monkey, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μgEHD3 monoclonal antibody (M01), clone 4B7 [orb2291189]
ELISA, IF, IHC-P, WB
Human
Mouse
Monoclonal
Unconjugated
100 μg

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Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of EHD3 Antibody in mouse lung tissue lysates (35 ug/lane)

EHD3 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Protocol Information
EHD3 Antibody (orb1263206)
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