FOXA1 Antibody

SKU: orb750029

Description

FOXA1 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin as a pioneer factor. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.FOXA1 in breast cancer is highly correlated with ERa+, GATA3+, and PR+ protein expression as well as endocrine signaling. FOXA1 acts as a pioneer factor for ERa in ERa+ breast cancer, and its expression might identify ERa+ cancers that undergo rapid reprogramming of ERa signaling that is associated with poor outcomes and treatment resistance. Conversely, in ERa+/- breast cancer FOXA1 is highly correlated with low-grade morphology and improved disease free survival. FOXA1 is a downstream target of GATA3 in the mammary gland. Expression in ERa+/- cancers may identify a subset of tumors that is responsive to other endocrine therapies such as androgen receptor antagonist treatment. [Wiki]

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Developmental Biology

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the FOXA1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: FOXA1/1512
• Immunogen: A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the FOXA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This FOXA1 antibody is available for research use only.
• Disclaimer: For research use only

IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FOXA1/1512). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

IHC testing of FFPE human prostate with FOXA1 antibody (clone FOXA1/1512). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

Western blot testing of human 1) MCF7 (breast) and 2) PC3 (prostate) cell lysate with FOXA1 antibody (clone FOXA1/1512). Predicted molecular weight: ~49 kDa.

Western blot testing of human HeLa cell lysate with FOXA1 antibody (clone FOXA1/1512). Predicted molecular weight: ~49 kDa.

Immunofluorescent staining of PFA-fixed human MCF7 cells with FOXA1 antibody (clone FOXA1/1512, green) and Phalloiden (red).

FACS testing of PFA-fixed human MCF-7 cells with FOXA1 antibody (clone FOXA1/1512); Red = isotype control, Blue = FOXA1 antibody.

SDS-PAGE Analysis of Purified, BSA-Free FOXA1 Antibody (clone FOXA1/1512). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P55317

NCBI Gene ID

3169