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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution Range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FKHRL1. AA range:220-269 |
| Molecular Weight | 97 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−FoxO3A (phospho Ser253) rabbit pAb [orb768183]
ELISA, IF, IHC-P, WB
Human, Mouse, Rat, Zebrafish
Polyclonal
Unconjugated
100 μl, 50 μlFoxO1/3 (phospho Ser322/S325) rabbit pAb [orb768176]
ELISA, IHC-P, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl, 50 μl

Quality Guarantee
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Western Blot analysis of various cells using FoxO3A Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of Jurkat cells using FoxO3A Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of Mouse-heart (negtive control), HepG2, MCF7, HEK293 cell lysis using FoxO3A Polyclonal Antibody. Dylight 800, Goat Anti Rabbit IgG Secondary antibody was diluted at 1:10000.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FKHRL1 Antibody. The picture on the right is blocked with the synthesized peptide.
Protocol Information
FoxO3A rabbit pAb (orb765241)
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