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GDNF Antibody (biotin)

SKU: orb1272526

Description

GDNF Antibody (biotin)

Research Area

,Growth Factors

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Application Notes
ELISA:Sandwich:To detect hGDNF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human GDNF (XP-5143) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGDNF.Western Blot:To detect hGDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

Antibody TypePrimary Antibody
ClonalityPolyclonal
ImmunogenProduced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGDNF (human GDNF).
TargetGDNF
PurificationAnti-hGDNF specific antibody was purified by affinity chromatography and then biotinylated.
ConjugationUnconjugated

Storage & Handling

StorageGDNF antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles.
Form/AppearanceLyophilized
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ATF1, ATF2, HSCR3, HFB1-GDNFGlial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor, hGDNF

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP39905

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.05 mg
$ 740.00
Free Secondary Antibody (20 ul)0/0

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