GDNF Antibody: Biotin

SKU: orb2652577

This product can be custom made

Description

Rabbit Anti-Human GDNF Polyclonal

Research Area

,Neuroscience | Neurodegeneration | Parkinson's Disease | ALS Disease | Alzheimer's Disease

Images & Validation

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Tested Applications	ELISA, ICC, IF, IHC, WB
Dilution Range	WB (1:1000); optimal dilutions for assays should be determined by the user.
Reactivity	Human, Mouse, Rat
Application Notes	1 µg/mL of SPC-810 was sufficient for detection of GDNF in 15 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Human GDNF Protein, Full length (AA78-211)
Target	GDNF
Conjugation	Biotin

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
Concentration	1mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Glial cell line-derived neurotrophic factor Antibody, Astrocyte-derived trophic factor Antibody, Astrocyte derived trophic factor 1 Antibody, Glial cell derived neurotrophic factor Antibody, ATF 1 Antibody, ATF 2 Antibody, Glial Cell Line Derived Neurotrophic Factor Antibody, ATF Antibody, Atf Antibody, HSCR3 Antibody, Astrocyte derived trophic factor Antibody, ATF2 Antibody, GDNF_HUMAN Antibody, GDNF Antibody, Glial derived neurotrophic factor Antibody, ATF1 Antibody, gdnf Antibody, HFB1 GDNF Antibody, hGDNF Antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western blot analysis with anti-GDNF polyclonal antibody showing detection of human recombinant GDNF protein. This recombinant protein has a predicted molecular weight of 15.2 kDa. Block: 5% skim milk. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody at 1:5000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min in RT. Exposed 9.122 seconds.

Western blot analysis with anti-GDNF polyclonal antibody showing detection of GDNF protein in rat brain lysate (prepared in-house). Rat GNDF protein with signal and pro-peptide segments has a predicted molecular weight of 23.6 kDa (UniProt Q07731). Block: 5% skim milk. Primary Antibody: Rabbit Anti-GDNF Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min in RT. Exposed 7.299 seconds. NOTE secondary antibody shows some non-specific binding in the control, but not to the GNDF protein.