BHMT Antibody

SKU: orb19599

Description

Goat polyclonal to BHMT

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Kidney shows strong cytoplasm staining in PCT cells. Recommended concentration, 2-4ug/ml.WB: Approx 45kDa band observed in Human Liver, Mouse Liver and Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/ml.

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: BHMT
• Protein Sequence: EQQLKELFEKQK
• Molecular Weight: 44.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti BHMT antibody, anti betaine-homocysteine methyltransferase antibody

Quick Database Links

Gene Symbol

BHMT

NCBI Gene ID

635