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ORC3L Antibody

SKU: orb18313

Description

Goat polyclonal antibody to ORC3L

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 75kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 82.3kDa according to NP_862820.1 and NP_036513.2). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetORC3L
Protein SequenceQKTDHVARLTWGGC
Molecular Weight82.3; 82.3; 65.9
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti ORC3L antibody, anti origin recognition complex, subunit 3-like (yeast) antibody, anti LAT antibody, anti ORC3 antibody, anti LATHEO antibody, anti IMAGE50150 antibody, anti origin recognition complex, subunit 3 (yeast homolog)-like antibody, anti OTTHUMP00000040611 antibody, anti homolog of latheo, Drosophila antibody, anti RP3-486L4.1 antibody, anti origin recognition complex, subunit 3 antibody

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 500.00
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Free Secondary Antibody (20 ul)0/0

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