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ORC3L Antibody

SKU: orb1247172

Description

ORC3L Antibody

Research Area

,Cancer

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 82.3kDa according to NP_862820.1 and NP_036513.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: QKTDHVARLTWGGC
TargetORC3L
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ORC3L, origin recognition complex, subunit 3-like (yeast), LAT, ORC3, LATHEO, IMAGE50150, origin recognition complex, subunit 3 (yeast homolog)-like, OTTHUMP00000040611, homolog of latheo, Drosophila, RP3-486L4.1, origin recognition complex, subunit 3

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 880.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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