Goat Anti-Rabbit IgG (H&L) Antibody (Alkaline Phosphatase)

SKU: orb1236935

Description

Goat Anti-Rabbit IgG (H&L) Antibody (Alkaline Phosphatase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Rabbit
• Application Notes:
  Alkaline Phosphatase Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:2,000 - 1:8,000, Western Blot 1:500 - 1:2,000, Immunohistochemistry 1:200 - 1:1,000. (Optimal dilution should be determined by user).

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Key Properties

• Antibody Type: Secondary Antibody
• Isotype: IgG
• Immunogen: Rabbit IgG - whole molecule
• Purification: Alkaline Phosphatase Conjugated Affinity Purified anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Alkaline Phosphatase Conjugated Affinity Purified anti-Rabbit IgG can be stored vial at 4 ˚°C before opening. DO NOT FREEZE. This product is stable at 4 ˚°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.01% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (rAlbumin)
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only