RACGAP1/MgcRacGAP Antibody

SKU: orb18360

Description

Goat polyclonal antibody to RACGAP1

Research Area

Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration, 1ug/ml.WB: Approx 60kDa band observed in Human Testis lysates (calculated MW of 71.0kDa according to NP_037409.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Toure et al., J Biol Chem 1998 Mar 13;273(11):6019-23; PMID: 9497316). Recommended concentration: 0.1-0.3 μg/ml.

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: RACGAP1 / MgcRacGAP
• Protein Sequence: GRQGNFFASPMLK
• Molecular Weight: 71.0; 63.9; 72.3; 61.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti RACGAP1 antibody, anti MgcRacGAP antibody, anti Rac GTPase activating protein 1 antibody, anti ID-GAP antibody, anti MGCRACGAP antibody, anti GTPase activating protein antibody, anti HsCYK-4 antibody, anti HsCYK-4 antibody

Quick Database Links

Gene Symbol

RACGAP1 / MgcRacGAP

NCBI Gene ID

29127