RACGAP1 Antibody

SKU: orb1247045

This product can be custom made

Description

RACGAP1 Antibody

Research Area

,Cancer

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC, IF, IHC, WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine
Application Notes	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell line Jurkat, and in nuclear lysates of cell lines A431 and Jurkat (calculated MW of 71.0kDa according to NP_037409.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.Immunohistochemistry:In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration: 1ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Polyclonal
Immunogen	The immunogen for this antibody is: C-GRQGNFFASPMLK
Target	RACGAP1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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RACGAP1, MgcRacGAP, Rac GTPase activating protein 1, ID-GAP, MGCRACGAP, GTPase activating protein, HsCYK-4, HsCYK-4

Quality Guarantee

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Quick Database Links

Gene Symbol

RACGAP1

UniProt

Q9H0H5

RefSeq (Protein):NP_001306935.1, NP_001306934.1, NP_037409.2, NP_001306936.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001306935.1, NP_001306934.1, NP_037409.2, NP_001306936.1

Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Applications:	ELISA, FC, IF, IHC, WB
Predicted Reactivity:	Bovine, Canine
Reactivity:	Human
Species/Host:	Goat
Clonality:	Polyclonal
Conjugation:	Unconjugated

Reactivity:	Human
Dynamic Range:	0.16-10 ng/mL
Sensitivity:	0.056 ng/mL

Applications:	ICC, IHC-P, WB
Reactivity:	Human, Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, WB
Reactivity:	Human
Species/Host:	Mouse
Clonality:	Monoclonal
Conjugation:	Unconjugated

Applications:	FC, IF, IHC-P, WB
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

RACGAP1 Antibody

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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