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Description
Research Area
Apoptotic, Cancer, Developmental Biology, Epigenetics, Neuroscience, Signaling Pathways
Images & Validation
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Item 1 of 3
| Tested Applications | ELISA, IF, WB |
|---|---|
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx. 37kDa band observed in Human Liver lysates and approx. 30+37kDa bands in Rat Liver lysates (calculated MW of 34.6kDa according to Human NP_001006611.1 and 31.1kDa according to Rat NP_543181.2 and 38.2kDa according to Rat XP_038953364.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS and HepG2 cells. Recommended concentration: 10µg/ml. |
| Reactivity | Human, Rat |
| Predicted Reactivity | Bovine, Canine, Mouse |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | SIAH1 |
| Protein Sequence | SRQTATALPTGTSKC |
| Molecular Weight | 31.1; 34.6 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti SIAH1 antibody, anti seven in absentia homolog 1 (Drosophila) antibody, anti Siah-1 antibody, anti hSIAH1 antibody, anti HUMSIAH antibody, anti Siah-1a antibody, anti FLJ08065 antibody, anti seven in absentia homolog 1 antibody, anti sonic hedgehog homolog antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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