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Description
Images & Validation
−| Tested Applications | SDS-PAGE |
|---|---|
| Application Notes |
Key Properties
−| Biological Origin | E.coli |
|---|---|
| Biological Activity | The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is 0.05 ng/ml. corresponding to Specific Activity of > 2.0 x 10,000,000 IU/mg. |
| Target | BTC |
| Conjugation | Unconjugated |
| Purity | > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Gly-Asn-Ser-Thr. |
Storage & Handling
−| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
|---|---|
| Buffer/Preservatives | The Betacellulin Human Recombinant was lyophilized after extensive dialysis against 20 mM phosphate buffer pH-7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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−Human BTC Protein, hFc Tag [orb1743320]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 35.1 kDa after removal of the signal peptide. The apparent molecular mass of BTC-hFc is approximately 35-70 kDa due to glycosylation.
E.coli
10 μg, 50 μg, 100 μgHuman BTC protein [orb382941]
Greater than 90% as determined by SDS-PAGE.
43.6 kDa
E.coli
20 μg, 100 μg, 1 mgHuman BTC protein [orb382991]
Greater than 90% as determined by SDS-PAGE.
18.6 kDa
Yeast
20 μg, 1 mg, 100 μg

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