Human Complement C5 Protein, His Tag

SKU: orb612115

Description

Human Complement C5 (w917s), His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # P01031-1 (W917S)).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 188.1 kDa. The protein migrates as 70 kDa and 120-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Human Complement C5 (w917s), His Tag at 2 µg/mL (100 µL/well) can bind Anti-C5a MAb (Human IgG1) with a linear range of 0.2-6 ng/mL (QC tested).
• Target: Complement C5
• Reactivity: Human
• Tag: C-10×His
• Expression Region: Gln 19 - Cys 1676
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 188.1 kDa
• Purity: 95%
• Protein Sequence: NP_001726.2

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Human Complement C5 (w917s), His Tag (Cat. No. orb612115) at 2 μg/mL (100 μL/well) can bind Anti-C5a MAb (Human IgG1) with a linear range of 0.2-6 ng/mL (QC tested).

Quick Database Links

Gene Symbol

Complement C5

UniProt

P01031

RefSeq (Protein):NP_001726.2