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Human Keratinocyte Growth Factor protein

SKU: orb80085

Description

Keratinocyte Growth Factor-1 Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18995 Dalton.The FGF-7 is purified by proprietary chromatographic techniques.

Images & Validation

Tested ApplicationsFA, HPLC, SDS-PAGE
Application Notes
< strong>Experiment Notes: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.9 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of KGF as Reference Standard.

Key Properties

Biological OriginE.coli
Biological ActivityThe ED50, calculated by the dose-dependant stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake) is 10ng/ml corresponding to specific activity of 100,000 Units/mg. Biorbyt's Keratinocyte Growth Factor biological activity was measured by the KGF's mitogenic activity on BaF3 cells expressing the KGF receptor.
TargetKGF
ConjugationUnconjugated
Purity> 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
Protein SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Asn-Asp-Met.

Storage & Handling

StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Buffer/PreservativesThe protein was lyophilized from concentrated (1mg/ml) solution with no additives.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

HBGF-7 protein, FGF7 protein, FGF-7 protein, KGF protein

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol
HPLC
High-Performance Liquid Chromatography
View Protocol

Available Sizes

Select a size below

10 μg
$ 340.00
100 μg
$ 1,480.00
500 μg
$ 3,300.00
DispatchUsually dispatched within 2-3 weeks
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