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Description
Research Area
Cytokines
Images & Validation
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Item 1 of 3
| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 |
|---|---|
| Biological Origin | Human |
| Biological Activity | Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
| Target | TNF-alpha |
| Reactivity | Human |
| Tag | Native |
| Expression Region | Val 77 - Leu 233 |
| Endotoxins | 0.01 EU per μg |
| Conjugation | Unconjugated |
| MW | 17.4 kDa |
| Purity | 95% |
| Protein Sequence | NP_000585.2 |
Customer Validated Data
− The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution
Latest Experiments
1 ResultsDilution
Sample
Summary
ELISA
Yeast
-
Yeast (Saccharomyces boulardii)
Human TNF-alpha Protein is used as...
Storage & Handling
−| Storage | -20°C to -70°C for 24 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
|---|---|
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000585.2 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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