Human TNF-beta Protein, His Tag

SKU: orb1733281

Description

Human TNF-beta Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 35 - Leu 205 (Accession # P01374).

Research Area

Cytokines

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.5 kDa. The protein migrates as 26-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Human TNFR1, Fc Tag at 5 µg/mL (100 µL/well) can bind Human TNF-beta Protein, His Tag with a linear range of 1-20 ng/mL (QC tested).
• Target: TNF-beta
• Reactivity: Human
• Tag: C-10×His
• Expression Region: Leu 35 - Leu 205
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 20.5 kDa
• Purity: 90%

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Human TNFR1, Fc Tag (Cat. No. orb257878) at 5 μg/mL (100 μL/well) can bind Human TNF-beta Protein, His Tag (Cat. No. orb1733281) with a linear range of 1-20 ng/mL (QC tested).

Quick Database Links

Gene Symbol

TNF-beta

UniProt

P01374