KRT7 Antibody / Cytokeratin 7

SKU: orb2638733

Description

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: KRT7/760
• Immunogen: Recombinant full-length human KRT7 protein was used as the immunogen for the Cytokeratin 7 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Cytokeratin 7 antibody is available for research use only.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 7 antibody (clone KRT7/760).

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 7 antibody (clone KRT7/760).

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Cytokeratin 7 antibody (clone KRT7/760).

Western blot testing of human HeLa cell lysate with Cytokeratin 7 antibody (clone KRT7/760). Predicted molecular weight ~51 kDa.

Flow cytometry testing of PFA-fixed human HeLa cells with Cytokeratin 7 antibody (clone KRT7/760); Red = isotype control, Blue = recombinant Cytokeratin 7 antibody.

Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody (clone KRT7/760, green) and Reddot nuclear stain (red).

Quick Database Links

UniProt

P08729