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KRT7 Antibody / Cytokeratin 7

SKU: orb749621

Description

CK7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Expressed in cultured epidermal, bronchial and mesothelial cells. [UniProt]

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Infectious Diseases

Images & Validation

Tested ApplicationsFACS, IF, IHC-P, WB
Dilution RangeWestern blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
ReactivityHuman
Application Notes
The stated application concentrations are suggested starting amounts. Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. View the recombinant version of this Cytokeratin 7 antibody.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.OV-TL12/30
ImmunogenOTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the Cytokeratin 7 antibody.
PurityProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageStore the Cytokeratin 7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
Expiration Date12 months from date of receipt.
Hazard InformationThis Cytokeratin 7 antibody is available for research use only.
DisclaimerFor research use only

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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
View Protocol
IF
Immunofluorescence
View Protocol

Available Sizes

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20 μg
$ 410.00
Free Secondary Antibody (20 ul)0/0

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