KRT7 Antibody / Cytokeratin 7

SKU: orb749621

Description

CK7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Expressed in cultured epidermal, bronchial and mesothelial cells. [UniProt]

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Infectious Diseases

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The stated application concentrations are suggested starting amounts. Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. View the recombinant version of this Cytokeratin 7 antibody.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: OV-TL12/30
• Immunogen: OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the Cytokeratin 7 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the Cytokeratin 7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Cytokeratin 7 antibody is available for research use only.
• Disclaimer: For research use only

Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody.

IHC: Formalin-fixed, paraffin-embedded ovarian carcinoma stained with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC: Formalin-fixed, paraffin-embedded human lung SCC stained with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Flow cytometry testing of PFA-fixed human HeLa cells with Cytokeratin 7 antibody (clone OV-TL12/30); Red = isotype control, Blue = recombinant Cytokeratin 7 antibody.

Western blot testing of human HeLa cell lysate with Cytokeratin 7 antibody (clone OV-TL12/30).

SDS-PAGE analysis of purified, BSA-free Cytokeratin 7 antibody (clone OV-TL12/30) as confirmation of integrity and purity.

Quick Database Links

UniProt

P08729