Lamin B1

SKU: orb1294412

Description

Rabbit polyclonal antibody to Lamin B1

Research Area

,Product Categories/Antibodies,Loading control

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: Western Blot 1:1000-1:3000 Immunofluorescence 1:200-1:500
• Reactivity: Human, Mouse, Rabbit

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Target: Lamin B1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: Anti-Lamin B1 antibody - at 1/2000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 7.5% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294412 overnight at 4°C.

A549 cells'cytoplasmic and nuclear proteins extracted by 30-min. A. SDS-PAGE gel shows the profiles of extracted cytoplasmic proteins and nuclear proteins. B. The proteins from the gel were transferred to nitrocellulose membrane and probed with Lamin B1 antibody, Histone H3 antibody (nuclear marker) and GAPDH antibody (cytoplasmic marker). Nuclear Protein Extraction Kit.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294412 anti-Lamin B1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

Lamin B1