LCK Antibody (N-term)

SKU: orb38424

Description

Purified rabbit polyclonal antibody.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Infectious Diseases,Primary Antibodies;Immunology;Signal Transduction;Anti-Kinome Antibodies;Viral process;Host-virus interaction;T cell activation;T cell receptor signaling

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100, IF/ICC: 1:10-50, FACS: 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB3088
• Immunogen: Synthetic Peptide
• Target: LCK
• Molecular Weight: 58001
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-"Proto-oncogene tyrosine-protein kinase LCK antibody, anti-P56-LCK antibody, anti-T cell-specific protein-tyrosine kinase" antibody

LCK Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of LCK Antibody (N-term) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of lysates from mouse thymus and rat thymus tissue lysate (from left to right), using LSK Antibody (I37). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

LCK

UniProt

P06239

RefSeq (Protein):NP_001036236.1, NP_005347.3