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−Item 1 of 3
| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 7F5E9G9 |
| Immunogen | Purified recombinant fragment of human MAP2K2 expressed in E. Coli. |
| Target | MAP2K2 |
| Molecular Weight | 44kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Ascitic fluid containing 0.03% sodium azide. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−MEK2; MKK2; MAPKK2
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Western blot analysis of PC-12 (lane1), Jurkat (lane2), Hela (lane3) and NIH/3T3 (lane4) cell lysate using MAP2K2 antibody.
Immunofluorescence analysis of Hela cells using MAP2K2 antibody
Flow cytometric analysis of Hela cells negative control (purple) us
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ICC
Immunocytochemistry
MAP2K2 (orb69230)
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