MCP-1/MCAF Antibody (biotin)

SKU: orb1272401

Description

MCP-1/MCAF Antibody (biotin)

Research Area

,Chemokines & Cytokines

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Rat
• Application Notes:
  ELISA:Sandwich:To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat MCP-1(MCAF) (XP-5219) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1(MCAF). Western Blot:To detect rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat MCP-1(MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat MCP-1(MCAF) (rat MCP-1(MCAF)).
• Target: Ccl2
• Purification: Anti-rat MCP-1(MCAF) specific antibody was purified by affinity chromatography and then biotinylated.
• Conjugation: Unconjugated

Storage & Handling

• Storage: MCP-1/MCAF antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MCP-1, Scya2, Sigje, Je, Mcp1, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, MCP-1

To detect Rat MCP-1 (MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Rat MCP-1 (MCAF) (orb1272402) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat MCP-1 (MCAF).

To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

Ccl2

UniProt

P14844

RefSeq:P14844