MCP-1/MCAF Antibody (biotin)

SKU: orb1272403

Description

MCP-1/MCAF Antibody (biotin)

Research Area

,Chemokines & Cytokines

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Sandwich:To detect hMCP-1/MCAF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human MCP-1/MCAF (XP-5218) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-1/MCAF.Western Blot:To detect hMCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1(MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-1(MCAF) (human Macrophage Chemoattractant Protein-1).
• Target: CCL2
• Purification: Anti-hMCP-1(MCAF) specific antibody was purified by affinity chromatography and then biotinylated.
• Conjugation: Unconjugated

Storage & Handling

• Storage: MCP-1/MCAF antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HSMCR30, C-C motif chemokine 2, HC11

To detect hMCP-1/MCAF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human MCP-1/MCAF (orb1272404) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMCP-1/MCAF.

Quick Database Links

Gene Symbol

CCL2

UniProt

P13500

RefSeq:P13500