Mineralocorticoid Receptor Antibody / MR / NR3C2

SKU: orb1823376

Description

Mineralocorticoid hormones are primarily found in epithelial tissues where they function as regulators of Na+, K+ and H+ ion transport. Aldosterone is a mineralocorticoid that has been shown to regulate electrolyte excretion and intravascular volume and is therefore involved in blood pressure regulation. Mineralocorticoid receptor (MCR or MR) is a member of the steroid/thyroid/ retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor downregulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: NR3C2/4900
• Immunogen: A recombinant partial protein sequence (within amino acids 601-673) from the human protein was used as the immunogen for the Mineralocorticoid Receptor antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the Mineralocorticoid Receptor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Mineralocorticoid Receptor antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human kidney tissue with Mineralocorticoid Receptor antibody (clone NR3C2/4900). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Mineralocorticoid Receptor antibody (clone NR3C2/4900) as confirmation of integrity and purity.

Quick Database Links

UniProt

P08235