Mineralocorticoid Receptor Antibody / MR / NR3C2

SKU: orb1823378

Description

Mineralocorticoid hormones are primarily found in epithelial tissues where they function as regulators of Na+, K+ and H+ ion transport. Aldosterone is a mineralocorticoid that has been shown to regulate electrolyte excretion and intravascular volume and is therefore involved in blood pressure regulation. Mineralocorticoid receptor (MCR or MR) is a member of the steroid/thyroid/ retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor downregulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

Images & Validation

• Tested Applications: FACS
• Dilution Range: Flow cytometry: 1-2ug/million cells
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: PCRP-NR3C2-1E3
• Immunogen: A recombinant partial protein sequence (within amino acids 601-673) from the human protein was used as the immunogen for the NR3C2 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the NR3C2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This NR3C2 antibody is available for research use only.
• Disclaimer: For research use only

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using NR3C2 antibody (PCRP-NR3C2-1E3). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Flow cytometry testing of PFA-fixed human HeLa cells with NR3C2 antibody (clone PCRP-NR3C2-1E3) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

SDS-PAGE analysis of purified, BSA-free NR3C2 antibody (clone PCRP-NR3C2-1E3) as confirmation of integrity and purity.

Quick Database Links

UniProt

P08235