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Mouse FGF2 protein

SKU: orb391730

Description

Recombinant mouse FGF2 protein is produced by our E. coli. It is a single non-glycosylated polypeptide chain containing 154 amino acids with a molecular mass of 17153 Daltons.

Research Area

Product Categories/Products/Proteins,Product Categories/Research Area,Epigenetics,Product Categories/Proteins,Developmental Biology,Cancer

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
TargetFGF2
ReactivityMouse
EndotoxinsEndotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Protein SequenceMAASGITSLP ALPEDGGAAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHVK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTEEC FFFERLESNN YNTYRSRKYS SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM PB, 400mM NaCl, pH 7.0.
Buffer/PreservativesLyophilized from a 0.2 μM filtered solution of 20mM PB, 400mM NaCl, pH 7.0.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

FGFB; Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

Select a size below

10 μg
$ 150.00
50 μg
$ 360.00
500 μg
$ 1,500.00
DispatchUsually dispatched within 5-10 working days
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