Murine BD3 protein

SKU: orb82606

Description

Beta defensin 3, also known as BD3 and DEFB3, is a membrane active cationic peptide that functions in inflammation and innate immune responses and coded by Defb 3 gene on chromosome 8 in mouse. There are at least 30 beta-defensins which are distinguished from alpha-defensins by the connectivity pattern of their three intramolecular disulfide bonds. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is upregulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds. BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 productions and inhibits TIMP1 and 2 expressions.

Images & Validation

• Tested Applications: HPLC, SDS-PAGE
• Application Notes:
  Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Key Properties

• Biological Origin: E.coli
• Biological Activity: Exhibits antimicrobial activity against gram-positive bacteria S. aureus and gram-negative P. aeruginosa and E.coli. Less than 1EU/μg of rMuBD-3 as determined by LAL method.
• Target: BD3
• Conjugation: Unconjugated
• MW: 4.6 kDa
• Purity: > 95% pure by SDS-PAGE and HPLC analyses.
• Protein Sequence: KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K

Storage & Handling

• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Sterile Filtered White lyophilized (freeze-dried) powder. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of Murine BD3 protein

Quick Database Links

Gene Symbol

BD3