N-Cadherin Antibody / CDH2

SKU: orb2637239

Description

N-Cadherin/CDH2/Cadherin-2 acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant N-Cadherin antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG
• Clone No.: CDH2/6857R
• Immunogen: Recombinant full-length human CDH2 protein was used as the immunogen for the recombinant N-Cadherin antibody.
• Purity: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This recombinant N-Cadherin antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human ovarian carcinoma tissue with recombinant N-Cadherin antibody (clone CDH2/6857R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of intercalated discs in FFPE human heart tissue with recombinant N-Cadherin antibody (clone CDH2/6857R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot analysis of human heart tissue lysate using recombinant N-Cadherin antibody (clone CDH2/6857R). Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).

Quick Database Links

UniProt

P19022