N-Cadherin Antibody / CDH2

SKU: orb2640214

Description

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CDH2 and CD325. CDH2 is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CDH2 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, the protein has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, it is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human, Mouse
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDH2 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: 8C11
• Immunogen: A human partial recombinant protein corresponding to the N-Cadherin extracellular domain was used as the immunogen for this CDH2 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CDH2 antibody is available for research use only.
• Disclaimer: For research use only

Western blot testing of human brain lysate with CDH2 antibody. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).

IHC testing of FFPE mouse heart tissue with CDH2 antibody. Required HIER: boil tissue sections in 10mM Tis with pH9 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human heart tissue lysate with CDH2 antibody. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).

Quick Database Links

UniProt

P19022