Ornithine Decarboxylase Antibody / ODC1

SKU: orb248486

Description

This antibody recognizes a 53kDa protein, identified as the Ornithine Decarboxylase. ODC-1 is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC-1 mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. Activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT (1) (2)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: ODC1/485
• Immunogen: Recombinant human ODC-1 protein was used as the immunogen for this antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the ODC-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This ODC-1 antibody is available for research use only.
• Disclaimer: For research use only

Formalin/paraffin human prostate carcinoma stained with ODC-1 antibody (clone ODC1/485).

Western blot testing of human placenta lysate using ODC-1 antibody (clone ODC1/485).

Flow analysis of permeablized PC3 cells using ODC-1 antibody (clone ODC1/485).

Immunofluorescence testing of LNCaP cells and Alexa Fluor 488 conjugated ODC-1 antibody (clone ODC1/485).

Formalin/paraffin rat pancreas stained with ODC-1 antibody (clone ODC1/485).

Formalin/paraffin human testicular carcinoma stained with ODC-1 antibody (clone ODC1/485).

Formalin/paraffin mouse pancreas stained with ODC-1 antibody (clone ODC1/485).

SDS-PAGE analysis of purified, BSA-free ODC-1 antibody (clone ODC1/485) as confirmation of integrity and purity.

Western blot testing of human PC3 cell lysate using ODC-1 antibody (clone ODC1/485).

Quick Database Links

NCBI Gene ID

4953