Ornithine Decarboxylase Antibody / ODC1

SKU: orb749726

Description

Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the ODC antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: ODC1/486
• Immunogen: Recombinant human protein was used as the immunogen for the ODC antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the ODC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This ODC antibody is available for research use only.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human pancreas stained with ODC antibody (ODC1/486)

IHC: Formalin-fixed, paraffin-embedded mouse pancreas stained with ODC antibody (ODC1/486)

IHC: Formalin-fixed, paraffin-embedded rat pancreas stained with ODC antibody (ODC1/486)

IHC: Formalin-fixed, paraffin-embedded mouse small intestine stained with ODC antibody (clone ODC1/486).

Western blot testing of human PC-3 cell lysate with ODC antibody. Predicted molecular weight ~51 kDa.

SDS-PAGE Analysis of Purified, BSA-Free ODC Antibody (clone ODC1/486). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P11926