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Description
Research Area
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | PD-L1 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 51.7 kDa and the accession number is Q9NZQ7-1. |
| Tag | C-mFc |
| Expression Region | A DNA sequence encoding the human CD274 (NP_054862.1) (Met1-Thr239) was expressed with the Fc region of mouse IgG1 at the C-terminus. Predicted N terminal: Phe 19 |
| MW | 51.7 kDa (predicted); 66 kDa (reducing condition, due to glycosylation) |
| Purity | 98.00% |
Storage & Handling
−| Storage | -20°C |
|---|---|
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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−Human PD-L1 Protein, mFc-His tag [orb689384]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.
Mammalian
100 μg, 10 μg, 50 μgRecombinant human PD-L1 protein, C-mFc (HEK293) [orb1516351]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 63-68 kDa based on Tris-Bis PAGE result. kDa
100 μgHuman CD274 protein [orb706872]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
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50 μg, 500 μg, 10 μg

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