Rabbit anti-Sp1 Antibody, Affinity Purified

SKU: orb1530644

Description

Sp1 Antibody

Research Area

Product Categories/Products/Antibodies,Primary Antibodies

Images & Validation

• Tested Applications: ChIP, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 735 and C-terminus
• Target: Sp1
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

specificity protein 1; transcription factor Sp1

Detection of human SP1 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, MCF-7, and CCRF-CEM cells. Antibody: Affinity purified rabbit anti-SP1 antibody at 0.1 µg/ml.

Detection of mouse SP1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5, 000 (0.2µg/ml).

Detection of human SP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SP1 used at a dilution of 1:5, 000 (0.2µg/ml).

Detection of human SP1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-SP1 antibody.

Localization of Sp1 Binding Sites by ChIP-sequencing. Chromatin from acute myeloid leukemia cell line was immunoprecipitated with anti-Sp1 antibody and analyzed by DNA sequencing.

Quick Database Links

Gene Symbol

Sp1

UniProt

P08047

RefSeq:XP_028606.4