Rabbit anti-Sp1 Antibody, Affinity Purified

SKU: orb1530645

Description

Sp1 Antibody

Research Area

Product Categories/Products/Antibodies,Primary Antibodies

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:10,000 - 1:25,000; IP - 1 - 4 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 700 and 750
• Target: Sp1
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

specificity protein 1; transcription factor Sp1

Detection of human SP1 by western blot.Samples: Whole cell lysate from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-SP1 antibody orb1530645 at 0.04 µg/ml.

Detection of human and mouse SP1 by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:1, 000 (1 µg/ml).

Detection of human SP1 by WB and IP.Samples: A. HEK HEK293T cells. B. HEK 293T cells. Antibody: A. Affinity purified rabbit anti-Sp1 antibody orb1530645 used at the indicated concentrations.

Quick Database Links

Gene Symbol

Sp1

UniProt

P08047

RefSeq:XP_028606.4