RAN Antibody (N-term)

SKU: orb1929880

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Cell Biology;Signal Transduction;Viral process;Host-virus interaction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Reactivity: Human, Mouse
• Predicted Reactivity: C. elegans, Other, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB20229
• Target: This RAN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human RAN.
• Molecular Weight: 24423 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GTP-binding nuclear protein Ran, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein, RAN, ARA24

RAN Antibody (N-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of RAN Antibody (N-term) in HL-60 cell line lysates (35 ug/lane).RAN (arrow) was detected using the purified Pab.

Western blot analysis of RAN antibody (N-term) in NIH-3T3 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified Pab.

Western blot analysis of RAN (arrow) using rabbit polyclonal RAN Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.

Quick Database Links

Gene Symbol

This RAN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human RAN.

UniProt

P62826

RefSeq (Protein):NP_006316.1